The 27th International Meeting of the European Light Microscopy Initiative (ELMI 2027) will bring together leading researchers, core facility managers, technology developers, industry representatives and innovators working in biological and biomedical imaging. ELMI has become one of the most important European meetings dedicated to advanced light microscopy, imaging technologies and their applications in life sciences. The congress offers a unique environment where academia and industry meet to exchange knowledge, present new technologies and create long-term collaborations.